Journal: Redox Biology

Article Title: Loss of Selenov predisposes mice to extra fat accumulation and attenuated energy expenditure

doi: 10.1016/j.redox.2021.102048

Figure Lengend Snippet: Effects of Selenov knockout on body weight, tissue fat accumulation, adipocyte histology, and serum lipid profiles and related biomarkers in male mice fed a normal-fat (control) or a high-fat diet from 2- to 6-month old of age. (A) Body weight (n = 10). (B) Upper panel images: magnetic resonance analysis of total body fat mass. Both the vertical (top) and cross (bottom) sections showed increased fat (red color) accumulations by KO and high-fat diet. Lower bar graphs: weights of total fat and individual adipose tissues (n = 10). (C) H&E staining of paraffin-embedded EWAT sections: adipocyte hypertrophy and enlarged lipid droplets by the KO and high-fat diet, scale bar, 20 μm. The image was a representative of five sets of data. (D) Serum concentrations of TC, TG, NEFA, leptin, TNF α , IL6, Mcp1, and adiponectin (n = 6). All graphic data are mean ± SE. Means without sharing a common superscript letter are different (P < 0.05). Abbreviations: BAT, brown adipose tissue; EWAT, epididymis white adipose tissue; HF, high-fat diet; IL6, interleukin 6; KO, knockout; Mcp1, monocyte chemoattractant protein-1; NEFA, non-esterified fat acid; NF, normal-fat diet; TC, total cholesterol; TG, total triglyceride; TNF α , tumor necrosis factor alpha; WT, wild-type. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Respective commercial kits were also used to determine serum concentrations of adiponectin and leptin (Crystal Chem, Downers Grove, IL, USA), interleukin 6 (IL-6) and tumor necrosis factor α (TNFα) (eBioscience, San Diego, CA, USA), and monocyte chemoattractant protein-1 (Mcp1) (BioLengend, San Diego, CA, USA).

Techniques: Knock-Out, Staining